1WE1
Crystal structure of heme oxygenase-1 from cyanobacterium Synechocystis sp. PCC6803 in complex with heme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-01-29 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 110.790, 113.730, 109.700 |
Unit cell angles | 90.00, 112.26, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.224 |
Rwork | 0.220 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dve |
RMSD bond length | 0.008 |
RMSD bond angle | 1.214 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 46584 | |
<I/σ(I)> | 7.2 | 2.4 |
Completeness [%] | 94.7 | 83.2 |
Redundancy | 3.3 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | PEG 400, 2-propanol, 1,5-diaminopentane dihydrochloride, sodium citrate, pottasium phosphate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |