1WE0
Crystal structure of peroxiredoxin (AhpC) from Amphibacillus xylanus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 290 |
Detector technology | IMAGE PLATE |
Detector | FUJI |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 79.216, 79.319, 104.449 |
Unit cell angles | 77.22, 82.33, 80.08 |
Refinement procedure
Resolution | 50.000 - 2.900 |
Rwork | 0.208 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 46092 | |
Completeness [%] | 83.6 | 55.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG 6000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG 6000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |