1WDW
Structural basis of mutual activation of the tryptophan synthase a2b2 complex from a hyperthermophile, Pyrococcus furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | OXFORD PX210 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.056, 220.257, 292.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.170 | |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.091 | 0.367 |
Number of reflections | 115486 | |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | sodium citrate, PEG 8000, potassium acetate, ethylene glycol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |