1WDU
Endonuclease domain of TRAS1, a telomere-specific non-LTR retrotransposon
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6B |
Synchrotron site | Photon Factory |
Beamline | BL-6B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-10-14 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.000 |
Spacegroup name | P 32 |
Unit cell lengths | 64.645, 64.645, 117.315 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
Rwork | 0.225 |
R-free | 0.25300 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.230 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.084 | 0.182 |
Number of reflections | 21413 | |
<I/σ(I)> | 5.2 | 2.2 |
Completeness [%] | 99.7 | 100 |
Redundancy | 5.8 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 283 | ammonium sulfate, IPA, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 283K |