1WDP
The role of an inner loop in the catalytic mechanism of soybean beta-amylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2003-11-29 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.71 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 84.888, 84.888, 143.397 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.270 |
R-factor | 0.134 |
R-free | 0.17370 |
Structure solution method | AB INITIO |
RMSD bond length | 0.014 |
RMSD bond angle | 0.030 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.290 |
High resolution limit [Å] | 1.250 | 1.250 |
Number of reflections | 163644 | |
Completeness [%] | 99.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 277 | ammonium sulfate, sodium acetate, 2-mercaptoethanol, EDTA, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |