1WDN

GLUTAMINE-BINDING PROTEIN

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-01-25
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	66.700, 91.000, 34.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.940
R-factor	0.2
Rwork	0.200
R-free	0.30000
RMSD bond length	0.014
RMSD bond angle	1.450
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.900 *	1.940 *
Rmerge	0.082
Total number of observations	65773 *
Number of reflections	15110
Completeness [%]	91.1	82.5 *
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	PEG4000	15 (%(w/v))
3	1	drop		100 (mM)
4	1	drop	sodium acetate	0.1 (M)