1WDM
fatty acid beta-oxidation multienzyme complex from Pseudomonas fragi, form I (native3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.453, 95.462, 161.325 |
| Unit cell angles | 90.00, 111.79, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.800 |
| R-factor | 0.2171 |
| Rwork | 0.214 |
| R-free | 0.26170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | form I |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.978 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.010 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.124 | 0.236 |
| Number of reflections | 24902 | |
| <I/σ(I)> | 5.9 | 3.2 |
| Completeness [%] | 98.0 | 98.5 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG4000, MES, ZnCl2, n-octylpentaoxyethylene, NAD+, acetoacetyl-CoA, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
