1WDD

Crystal Structure of Activated Rice Rubisco Complexed with 2-Carboxyarabinitol-1,5-bisphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-15
Detector	Bruker DIP-6040
Wavelength(s)	0.90000
Spacegroup name	I 4
Unit cell lengths	111.713, 111.713, 196.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.320 - 1.350
R-factor	0.16
Rwork	0.160
R-free	0.18200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ir1
RMSD bond length	0.022
RMSD bond angle	1.900
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS (1.1)

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	VAPOR DIFFUSION, HANGING DROP, temperature 293K