1WD6
crystal structure of JW1657 from Escherichia coli
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-27 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.9640, 0.97914, 0.97964 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 85.803, 85.803, 95.923 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.370 - 2.900 |
Rwork | 0.208 |
R-free | 0.24200 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 9408 | |
Completeness [%] | 99.7 | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Oil Batch | 7 | 293 | Ammonium Sulfate, PEG8000, pH 7.0, Oil Batch, temperature 293K |