1WD1
Crystal structures of the hyperthermophilic chromosomal protein Sac7d in complex with DNA decamers
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-07-18 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.634, 46.841, 53.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.25 |
Rwork | 0.248 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdb 1azp |
RMSD bond length | 0.020 |
RMSD bond angle | 2.170 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.065 | 0.540 |
Number of reflections | 5166 | |
<I/σ(I)> | 25 | 2 |
Completeness [%] | 98.0 | 88.5 |
Redundancy | 4.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | potassium phosphate, PEG8000, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | potassium phosphate | ||
2 | 1 | 1 | PEG8000 | ||
3 | 1 | 1 | H2O | ||
4 | 1 | 2 | potassium phosphate | ||
5 | 1 | 2 | PEG8000 | ||
6 | 1 | 2 | H2O |