1WCZ
Crystal structure of an alkaline form of v8 protease from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 61.290, 61.290, 221.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 2.000 |
| R-factor | 0.2121 |
| Rwork | 0.205 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qy6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.139 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 223.600 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.073 | 0.247 |
| Number of reflections | 17429 | |
| <I/σ(I)> | 9.2 | 2.2 |
| Completeness [%] | 99.2 | 99.3 |
| Redundancy | 9.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.6 | 283 | PEG-MME 5000, sodium chloride, ammonium chloride, CAPS buffer, pH 10.6, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
