1WCW

Crystal Structure of Uroporphyrinogen III Synthase from an Extremely Thermophilic Bacterium Thermus thermophilus HB8 (Wild type, Native, Form-1 crystal)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-12-11
Detector	RIGAKU RAXIS V
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	35.546, 66.309, 55.677
Unit cell angles	90.00, 91.78, 90.00

Refinement procedure

Resolution	35.530 - 1.300
Rwork	0.188
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.900
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.350
High resolution limit [Å]	1.300	1.300
Completeness [%]	91.8	80

Crystallization Conditions

crystal ID	method	pH	temperature	details
1