1WCU
CBM29_1, A Family 29 Carbohydrate Binding Module from Piromyces equi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.054, 57.512, 76.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.130 - 1.500 |
| R-factor | 0.141 |
| Rwork | 0.140 |
| R-free | 0.16400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gwk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.410 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.120 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.100 |
| Number of reflections | 21737 | |
| <I/σ(I)> | 40 | 13 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.6M LITHIUM ACETATE, 20% PEG 3350. WITH PROTEIN AT A FINAL CONCENTRATION OF 10 MG / ML FINAL. |
