1WCM
Complete 12-Subunit RNA Polymerase II at 3.8 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 222.718, 395.135, 284.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.800 |
| R-factor | 0.2569 |
| Rwork | 0.257 |
| R-free | 0.28540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.360 |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.950 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.070 | |
| Number of reflections | 121866 | |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 99.3 | 97 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
