1WBC

CRYSTALLIZATION AND PRELIMINARY X-RAY STUDIES OF PSOPHOCARPIN B1, A CHYMOTRYPSIN INHIBITOR FROM WINGED BEAN SEEDS

Experimental procedure

Spacegroup name	P 61 2 2
Unit cell lengths	61.400, 61.400, 210.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.950
R-factor	0.191
Rwork	0.191
RMSD bond length	0.015
RMSD bond angle	28.100 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.950 *
Rmerge	0.046 *
Total number of observations	9549 *
Number of reflections	3913 *
Completeness [%]	85.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6.67 (mg/ml)
2	1	drop	ammonium sulfate	8.3 (%)
3	1	drop	Tris-HCl	0.1 (M)
4	1	reservoir	ammonium sulfate	25 (%)
5	1	reservoir	Tris-HCl	0.1 (M)