1WBA
WINGED BEAN ALBUMIN 1
Experimental procedure
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 286 |
Detector technology | DIFFRACTOMETER |
Collection date | 1994-12 |
Detector | WEISSENBERG |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 95.600, 95.600, 86.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.800 |
R-factor | 0.19 |
Rwork | 0.190 |
Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT AND MOLECULAR REPLACEMENT |
Starting model (for MR) | SOYBEAN TRYPSIN INHIBITOR |
RMSD bond length | 0.008 |
RMSD bond angle | 24.874 * |
Data reduction software | Agrovata |
Data scaling software | Agrovata |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.092 |
Number of reflections | 12039 |
<I/σ(I)> | 4.2 |
Completeness [%] | 80.0 |
Redundancy | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 5.5 | VAPOR DIFFUSION FROM PROTEIN AT 5MG/ML IN 0.1M SUCCINATE BUFFER PH 5.5 EQUILIBRATED AGAINST 30% ETHANOL., vapor diffusion |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | ethanol | 30 (%) |