1WB8
Iron Superoxide Dismutase (FE-SOD) from the Hyperthermophile SULFOLOBUS SOLFATARICUS. 2.3 A Resolution Structure of Recombinant Protein with a Covalently Modified Tyrosine in the Active Site.
Replaces: 1SSSExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU ROTAFLEX RU-2 |
Temperature [K] | 294 |
Detector technology | IMAGE PLATE |
Collection date | 1997-07-09 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 76.266, 124.324, 60.268 |
Unit cell angles | 90.00, 128.75, 90.00 |
Refinement procedure
Resolution | 22.910 - 2.300 |
R-factor | 0.167 |
Rwork | 0.167 |
R-free | 0.18700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ABM |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.000 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.090 | 0.310 |
Number of reflections | 18647 | |
<I/σ(I)> | 7.4 | 2.3 |
Completeness [%] | 96.3 | 94.7 |
Redundancy | 4.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | CRYSTALS WERE GROWN BY VAPOR DIFFUSION IN HANGING DROPS AT 21C IN A 1 TO 1 MIX OF THE RESERVOIR SOLUTION (8% PEG 8000, 0.1 M TRIS/HCL PH 8.5) AND A PROTEIN SOLUTION (2.0 MG/ML SSSOD). |