1WAU
Structure of KDPG Aldolase E45N mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-03-15 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 105.195, 105.195, 51.444 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 91.290 - 2.800 |
R-factor | 0.174 |
Rwork | 0.171 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eua |
RMSD bond length | 0.028 |
RMSD bond angle | 2.311 |
Data reduction software | d*TREK |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0007) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.550 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.060 | 0.460 |
Number of reflections | 15069 | |
<I/σ(I)> | 8.5 | 1.6 |
Completeness [%] | 96.1 | 93.8 |
Redundancy | 1.7 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 0.1M AMMONIUM SULPHATE, 15% PEG4000, 10MM TRIS, PH8.0, pH 8.00 |