1WAR
Recombinant Human Purple Acid Phosphatase expressed in Pichia Pastoris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 300 |
Detector technology | IMAGE PLATE |
Collection date | 2003-08-20 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 80.538, 80.538, 99.998 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.980 - 2.220 |
R-factor | 0.141 |
Rwork | 0.138 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ute |
RMSD bond length | 0.013 |
RMSD bond angle | 1.373 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.980 | 2.300 |
High resolution limit [Å] | 2.220 | 2.220 |
Rmerge | 0.070 | 0.400 |
Number of reflections | 15951 | |
<I/σ(I)> | 17.97 | 5.49 |
Completeness [%] | 94.5 | 94 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 277 | THE PROTEIN (10 MG/ML) IN 50 MM MES AT PH 6, WAS PASSED THROUGH AN ULTRAFREE-MC 0.22 MM SPIN FILTER UNIT (MILLIPORE) AND ALLOWED TO STAND AT 4 DEGREES C. PURPLE CRYSTALS GREW SPONTANEOUSLY OVERNIGHT AND REACHED SIZES UP TO 0.5 MM IN SEVERAL WEEKS. |