1WAR

Recombinant Human Purple Acid Phosphatase expressed in Pichia Pastoris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	300
Detector technology	IMAGE PLATE
Collection date	2003-08-20
Detector	MAR scanner 345 mm plate
Spacegroup name	P 43 21 2
Unit cell lengths	80.538, 80.538, 99.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.980 - 2.220
R-factor	0.141
Rwork	0.138
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ute
RMSD bond length	0.013
RMSD bond angle	1.373
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.980	2.300
High resolution limit [Å]	2.220	2.220
Rmerge	0.070	0.400
Number of reflections	15951
<I/σ(I)>	17.97	5.49
Completeness [%]	94.5	94
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			277	THE PROTEIN (10 MG/ML) IN 50 MM MES AT PH 6, WAS PASSED THROUGH AN ULTRAFREE-MC 0.22 MM SPIN FILTER UNIT (MILLIPORE) AND ALLOWED TO STAND AT 4 DEGREES C. PURPLE CRYSTALS GREW SPONTANEOUSLY OVERNIGHT AND REACHED SIZES UP TO 0.5 MM IN SEVERAL WEEKS.