1WAL
3-ISOPROPYLMALATE DEHYDROGENASE (IPMDH) MUTANT (M219A)FROM THERMUS THERMOPHILUS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 78.400, 78.400, 158.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 2.270 |
R-factor | 0.184 * |
Rwork | 0.184 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ipd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.300 |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.270 |
Rmerge | 0.055 * |
Total number of observations | 54255 * |
Number of reflections | 22587 * |
Completeness [%] | 84.0 |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.6 | 6 MG/ML PROTEIN IN 1.4 M AMMONIUM SULFATE, pH 7.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 1.4 (M) | |
3 | 1 | drop | 20 (mM) |