1WAC
Back-priming mode of Phi6 RNA-dependent RNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 150 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.579, 105.851, 157.712 |
| Unit cell angles | 90.00, 98.86, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hi8 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.020 | |
| Number of reflections | 57385 | |
| <I/σ(I)> | 7.4 | 1.9 |
| Completeness [%] | 99.2 | 98.6 |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1M SODIUM CITRATE PH 5.6, 19% ISOPROPANOL, 19% PEG 4K, 5% GLYCEROL |
