1WA9
Crystal Structure of the PAS repeat region of the Drosophila clock protein PERIOD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 |
| Unit cell lengths | 140.440, 140.440, 61.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.150 |
| R-factor | 0.2279 |
| Rwork | 0.228 |
| R-free | 0.27720 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.511 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.250 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.050 | 0.350 |
| Number of reflections | 21048 | |
| <I/σ(I)> | 18.1 | 4.6 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.7 | 500 MM CALCIUM CHLORIDE, pH 7.70 |
