1W9Z
Structure of Bannavirus VP9
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.87, 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.386, 73.739, 95.722 |
| Unit cell angles | 90.00, 97.19, 90.00 |
Refinement procedure
| Resolution | 94.920 - 2.560 |
| R-factor | 0.18646 |
| Rwork | 0.183 |
| R-free | 0.24844 |
| Structure solution method | MAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (18.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.090 | 0.410 |
| Number of reflections | 27995 | |
| <I/σ(I)> | 10.6 | 2 |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M HEPES-NA, PH 7.5, 20% (W/V) POLYETHYLENE GLYCOL 4000, 10% (V/V) ISO-PROPANOL |
