1W9Y
The structure of ACC oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-21 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 70.300, 107.930, 108.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.100 |
| R-factor | 0.2237 |
| Rwork | 0.224 |
| R-free | 0.29210 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.350 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.200 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.050 | 0.300 |
| Number of reflections | 23601 | |
| <I/σ(I)> | 35.6 | 1.82 |
| Completeness [%] | 98.4 | 85.8 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10.3 | 1.2 M SODIUM DIHYDROPHOSPHATE 0.6 M DIPOTASSIUM HYDROPHOSPHATE 0.3 M LITHIUM SULPHATE 0.1 M CAPS PH 10.5 |
