1W9F
CBM29-2 mutant R112A: Probing the Mechanism of Ligand Recognition by Family 29 Carbohydrate Binding Modules
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 91.802, 91.802, 79.865 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.620 - 2.250 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gwm |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.697 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.060 | 0.570 |
| Number of reflections | 15514 | |
| <I/σ(I)> | 23.1 | 1.6 |
| Completeness [%] | 92.8 | 70.6 |
| Redundancy | 5.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM NA/HEPES BUFFER PH 7.5, 150 MM KSCN, 20% ETHYLENE GLYCOL, 18% PEG3350 |
