1W99
Mosquito-larvicidal toxin Cry4Ba from Bacillus thuringiensis ssp. Israelensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-15 |
Detector | ADSC CCD |
Spacegroup name | H 3 2 |
Unit cell lengths | 185.800, 185.800, 187.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 73.970 - 1.750 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.21800 |
Structure solution method | MIRAS |
Starting model (for MR) | 1dlc PDB ENTRY 1CIY |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MLPHARE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.970 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.066 | 0.353 |
Number of reflections | 1207111 | |
<I/σ(I)> | 24.1 | 15.1 |
Completeness [%] | 99.6 | 100 |
Redundancy | 9.7 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | VAPOUR DIFFUSION AT 20C IN HANGING DROPS CONTAINING 1:1 MIXTURE OF PROTEIN AT 8 MG/ML IN 50 MM SODIUM CARBONATE PH 10.5, 0.1 M SODIUM BROMIDE, 1 MM DTT AND PROTEASE INHIBITORS, AND THE RESERVOIR BUFFER CONSISTING OF 100MM TRIS ACETATE PH7-8 AND 0.4-0.6 M SODIUM BROMIDE. |