1W99
Mosquito-larvicidal toxin Cry4Ba from Bacillus thuringiensis ssp. Israelensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-15 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 185.800, 185.800, 187.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 73.970 - 1.750 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.21800 |
| Structure solution method | MIRAS |
| Starting model (for MR) | 1dlc PDB ENTRY 1CIY |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.970 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.066 | 0.353 |
| Number of reflections | 1207111 | |
| <I/σ(I)> | 24.1 | 15.1 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 9.7 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | VAPOUR DIFFUSION AT 20C IN HANGING DROPS CONTAINING 1:1 MIXTURE OF PROTEIN AT 8 MG/ML IN 50 MM SODIUM CARBONATE PH 10.5, 0.1 M SODIUM BROMIDE, 1 MM DTT AND PROTEASE INHIBITORS, AND THE RESERVOIR BUFFER CONSISTING OF 100MM TRIS ACETATE PH7-8 AND 0.4-0.6 M SODIUM BROMIDE. |
