1W97
cyto-EpsL: the cytoplasmic domain of EpsL, an inner membrane component of the type II secretion system of Vibrio cholerae
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-29 |
Wavelength(s) | 0.97951,0.97965,0.97951 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 61.006, 88.707, 55.371 |
Unit cell angles | 90.00, 106.01, 90.00 |
Refinement procedure
Resolution | 17.690 - 2.700 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.29000 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.740 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.054 | 0.369 |
Number of reflections | 7476 | |
<I/σ(I)> | 21.7 | 3.5 |
Completeness [%] | 95.8 | 75.8 |
Redundancy | 7 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 293 | 11-15% PEG 4000, 150MM CAOAC2, 100MM TRIS PH8, 20 DEGREES C, pH 8.00 |