1W93
Crystal Structure of Biotin Carboxylase Domain of Acetyl-Coenzyme A Carboxylase from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Spacegroup name | P 62 |
Unit cell lengths | 101.740, 101.740, 145.830 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.630 - 2.500 |
R-factor | 0.254 |
Rwork | 0.254 |
R-free | 0.32300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL |
Data scaling software | HKL |
Phasing software | COMO |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.080 | 0.250 |
Number of reflections | 24971 | |
<I/σ(I)> | 16.4 | 3.6 |
Completeness [%] | 84.3 | 57.4 |
Redundancy | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |