1W8P

Structural properties of the B25Tyr-NMe-B26Phe insulin mutant.

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	293
Detector technology	IMAGE PLATE
Spacegroup name	P 1 21 1
格子定数 [Å]	59.903, 62.116, 47.796
格子定数 [度]	90.00, 110.58, 90.00

精密化法

残基	25.570 - 2.080
R因子	0.192
Rwork	0.189
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1evr
結合長の平均二乗偏差(RMSD) [Å]	0.018
結合角の平均二乗偏差(RMSD) [度]	1.708
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	25.560	2.190
分解能 [Å] (高)	2.080	2.080
Rmerge_l_obs	0.030	0.120
独立反射数	17496
<I/σ(I)>	6
完全性 [%]	88.0	89
冗長性	5.5

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		8		0.1 M TRI-SODIUM CITRATE, 0.02 % W/V ZINC ACETATE, 6 % W/V TRIS/HCL PH 8.2, 0.1 % W/V PHENOL.