1W8G
CRYSTAL STRUCTURE OF E. COLI K-12 YGGS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-08 |
| Detector | ADSC CCD |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 124.017, 124.017, 83.729 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.000 - 2.000 |
| R-factor | 0.163 |
| Rwork | 0.161 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b54 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.119 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.340 |
| Number of reflections | 22295 | |
| <I/σ(I)> | 34.2 | 5.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 20% PEG 4000, 16% ISOPROPANOL, 0.1 M NA-CITRATE BUFFER PH 6, 5% SODIUM DEXTRANE SULPHATE |
