1W81

Crystal structure of apical membrane antigen 1 from Plasmodium vivax

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-21
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	54.097, 76.096, 103.925
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.010
R-factor	0.198
Rwork	0.194
R-free	0.25900
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.660
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.060	0.320
Number of reflections	147056
<I/σ(I)>	23.1	2.8
Completeness [%]	88.7	53.4
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		THE CRYSTALLISATION MIXTURE CONSISTED OF 10-12% (W/V) PEG3350, 100-200MM IMIDAZOLE, PH 7.0, 5-10% (V/V) ISOPROPANOL, AND 1% (V/V) DMF OR 3% (V/V) T-BUTANOL. THE PROTEIN SOLUTION WAS DILUTED BY 40 - 60% WITH THE CRYSTALLISATION BUFFER, GIVING A FINAL CONCENTRATION OF 4-6 MG. ML-1 IN SUSPENDED DROPS OF VOLUME 2 - 3 PERCENT.