1W7U
Photoproduct of the Wild-Type Aequorea victoria Green Fluorescent Protein after structural annealing at 170K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-08 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 155.273, 52.705, 141.746 |
Unit cell angles | 90.00, 119.91, 90.00 |
Refinement procedure
Resolution | 119.520 - 1.850 |
R-factor | 0.182 |
Rwork | 0.179 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hcj |
RMSD bond length | 0.013 |
RMSD bond angle | 1.521 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.150 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.080 | 0.262 |
Number of reflections | 85637 | |
<I/σ(I)> | 5.7 | 2.8 |
Completeness [%] | 97.3 | 84 |
Redundancy | 3.9 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.8 | 277 | CRYSTALS WERE GROWN AT 4C FROM 50 MM MGCL2, 14-17 % PEG3350 AND 50-100 MM TRIS/CL PH 7.8 - 8.6. |