1W7N
Crystal structure of human kynurenine aminotransferase I in PMP form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Detector | MSC |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 145.461, 145.461, 67.087 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.920 - 2.900 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w7l |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.660 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.096 * |
| Total number of observations | 110645 * |
| Number of reflections | 18243 |
| Completeness [%] | 99.3 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 4 * | pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | ammonium formate | 4.5 (M) | |
| 3 | 1 | reservoir | Tris | 0.1 (M) | pH7.5 |
