1W7B
Annexin A2: Does it induce membrane aggregation by a new multimeric state of the protein.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-24 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.352, 62.834, 119.017 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.380 - 1.520 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 1.550 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.050 | 0.910 |
Number of reflections | 59682 | |
<I/σ(I)> | 39 | 3 |
Completeness [%] | 95.6 | 88.1 |
Redundancy | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 2.5 M NACL 0.1 M ACETATE PH 4.5 0.2 M LI2SO4 |