1W7A
ATP bound MutS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-11-10 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.636, 93.040, 262.592 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.270 |
R-factor | 0.217 |
Rwork | 0.216 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e3m |
RMSD bond length | 0.013 |
RMSD bond angle | 1.294 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.080 | 0.770 |
Number of reflections | 99607 | |
<I/σ(I)> | 13.5 | 1.3 |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 7.5 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 12-14% PEG6000, 150-300MM NACL, 10 MM MGCL2, 25 MM HEPES PH 7.5, 100 UM ADP |