1W76
Orthorhombic form of Torpedo californica acetylcholinesterase (AChE) complexed with bis-acting galanthamine derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-03-19 |
| Detector | RIGAKU IMAGEPLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.223, 105.253, 150.559 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.170 - 2.300 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.28300 |
| Structure solution method | OTHER |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 63928 | |
| <I/σ(I)> | 11.5 | 2.7 |
| Completeness [%] | 98.4 | 98.9 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 277 | 35-40% W/V PEG 200, 0.1M MES PH5.8 4 DEG. CELSIUS |
