1W5H

An anti-parallel four helix bundle.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	93
Collection date	2004-06-26
Spacegroup name	P 42 21 2
Unit cell lengths	35.238, 35.238, 104.647
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.410 - 2.500
R-factor	0.282
Rwork	0.280
R-free	0.34400
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.410	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.050	0.360
Number of reflections	3786
<I/σ(I)>	17.2	4.7
Completeness [%]	100.0	100
Redundancy	7.06	7.11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.5		HANGING DROP, 1UL OF 1MG/ML PEPTIDE IN WATER, 1UL 100MM CAPS, 30% PEG 400, PH 10.5.