1W5G
An anti-parallel four helix bundle (acetimide modification).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Collection date | 2004-05-29 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 35.238, 35.238, 104.647 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.990 - 2.160 |
| R-factor | 0.305 |
| Rwork | 0.304 |
| R-free | 0.33700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.526 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.880 | 2.240 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.050 | 0.220 |
| Number of reflections | 3960 | |
| <I/σ(I)> | 17.3 | 6.5 |
| Completeness [%] | 100.0 | 97.6 |
| Redundancy | 5.37 | 5.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | HANGING DROP, 1UL OF 1MG/ML PEPTIDE IN WATER, 1UL 100MM CAPS, 30% PEG 400, PH 10.5. |
