1W5F
FtsZ, T7 mutated, domain swapped (T. maritima)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 181.510, 60.970, 66.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.2032 |
| Rwork | 0.203 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fsz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.181 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.220 |
| Number of reflections | 48484 | |
| <I/σ(I)> | 15.4 | 5.7 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 4 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
