1W58

FtsZ GMPCPP soak I213 (M. jannaschii)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Spacegroup name	I 21 3
Unit cell lengths	161.160, 161.160, 161.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.221
Rwork	0.221
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fsz
RMSD bond length	0.012
RMSD bond angle	1.612
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.650
High resolution limit [Å]	2.500	2.500
Rmerge	0.060	0.220
Number of reflections	19596
<I/σ(I)>	12.8	3.2
Completeness [%]	98.9	99.2
Redundancy	3.2	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1