1W4Q
Binding of Nonnatural 3'-Nucleotides to Ribonuclease A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-25 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.101, 32.925, 72.787 |
| Unit cell angles | 90.00, 90.39, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.680 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1afu |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.050 | 0.080 |
| Number of reflections | 27669 | |
| <I/σ(I)> | 25.7 | 12.5 |
| Completeness [%] | 94.8 | 87.6 |
| Redundancy | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 20MM SODIUM CITRATE, BUFFER PH 5.5, PEG 4000(25% W/V) |
