1W4P
Binding of Nonnatural 3'-Nucleotides to Ribonuclease A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-25 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.451, 32.942, 73.094 |
Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
Resolution | 41.390 - 1.690 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1afu |
RMSD bond length | 0.012 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.750 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.090 | 0.120 |
Number of reflections | 27984 | |
<I/σ(I)> | 9.4 | 8.3 |
Completeness [%] | 91.4 | 94.2 |
Redundancy | 3.2 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 20MM SODIUM CITRATE BUFFER, PH5.5, CONTAINING PEG4000 (25% W/V), pH 5.50 |