1W4C
P4 protein from Bacteriophage PHI12 apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 115.400, 126.300, 155.600 |
| Unit cell angles | 90.00, 91.60, 90.50 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.2514 |
| Rwork | 0.251 |
| R-free | 0.22360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w44 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.340 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.320 |
| Number of reflections | 291757 | |
| <I/σ(I)> | 18.6 | 3.9 |
| Completeness [%] | 96.0 | 77 |
| Redundancy | 9.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 10% PEG 1500, 100MM SODIUM ACETATE PH 4.8 PROTEIN CONCENTRATION 10 MG/ML, SITTING DROPS, 22C |
