1W4B
P4 protein from PHI12 in complex with product (AMPcPP Mg 22C)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-10 |
Detector | MARRESEARCH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 105.300, 129.300, 158.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.2363 |
Rwork | 0.236 |
R-free | 0.19640 |
Structure solution method | OTHER |
RMSD bond length | 0.011 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.080 | 0.310 |
Number of reflections | 44794 | |
<I/σ(I)> | 23 | 5.4 |
Completeness [%] | 93.0 | 100 |
Redundancy | 8.7 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 10% PEG 1500, 100MM SODIUM ACETATE PH 4.8 10MM AMPCPP 10MM MGCL, PROTEIN CONCENTRATION 10 MG/ML, SITTING DROPS, 22C |