1W46
P4 protein from Bacteriophage PHI12 in complex with ADP and MG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-10 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 105.000, 128.400, 158.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.2093 |
| Rwork | 0.209 |
| R-free | 0.25260 |
| Structure solution method | OTHER |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.320 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.140 | 0.330 |
| Number of reflections | 29666 | |
| <I/σ(I)> | 16 | 6.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 10% PEG 1500, 100MM SODIUM ACETATE PH 4.8 10MM ADP 10MM MGCL, PROTEIN CONCENTRATION 10 MG/ML, SITTING DROPS, 22C |
