1W44
P4 protein from Bacteriophage PHI12 in complex with ADP
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-24 |
Detector | ADSC CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 105.185, 130.908, 158.831 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.1823 |
Rwork | 0.182 |
R-free | 0.22770 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.580 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.090 | 0.620 |
Number of reflections | 68669 | |
<I/σ(I)> | 32 | 2.4 |
Completeness [%] | 93.0 | 97 |
Redundancy | 24 | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 10% PEG 1500, 100MM SODIUM ACETATE PH 4.8 10MM ADP, PROTEIN CONCENTRATION 10 MG/ML, SITTING DROP, 22C |