1W3Z
SeMet derivative of BbCRASP-1 from Borrelia Burgdorferi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 91.558, 91.558, 146.517 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.700 - 3.200 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.013 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | HYSS |
| Refinement software | TNT (5.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.300 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.090 | 0.420 |
| Number of reflections | 10849 | |
| <I/σ(I)> | 4 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.6 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 25% PEG600 200 MM IMIDAZOLE PH 6.0 5% GLYCEROL |
