1W3W
The 2.1 Angstroem resolution structure of annexin A8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 290 |
Detector technology | IMAGE PLATE |
Collection date | 2000-02-14 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.761, 65.581, 59.229 |
Unit cell angles | 90.00, 100.70, 90.00 |
Refinement procedure
Resolution | 23.310 - 1.990 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1axn |
RMSD bond length | 0.010 |
RMSD bond angle | 1.140 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.27) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.060 | 0.310 |
Number of reflections | 22712 | |
<I/σ(I)> | 21.7 | 5.52 |
Completeness [%] | 98.5 | 97.9 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 50MM CACODYLATE PH=6, 10MM CACL2, 45% (W/V) AMMONIUM SULPHATE |