1W3U

Crystal structure of phosphoserine aminotransferase from Bacillus circulans var. alkalophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-28
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	91.287, 91.109, 42.374
Unit cell angles	90.00, 111.25, 90.00

Refinement procedure

Resolution	15.000 - 1.500
R-factor	0.1421
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bt4
RMSD bond length	0.009
RMSD bond angle	0.028
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.030	0.200
Number of reflections	49679
<I/σ(I)>	20	3.8
Completeness [%]	96.0	91
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		CRYSTALLIZED AT ROOM TEMPERATURE FROM 0.1 M SODIUM ACETATE BUFFER, PH 4.6, 5% GLYCEROL, 4% PEG 20000