1W3O
Crystal structure of NimA from D. radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.863, 38.947, 59.811 |
| Unit cell angles | 90.00, 114.25, 90.00 |
Refinement procedure
| Resolution | 19.460 - 1.600 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.21100 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.709 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.600 | |
| Rmerge | 0.070 | 0.450 |
| Number of reflections | 26056 | |
| <I/σ(I)> | 11.2 | 2.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.65-0.9 M SODIUM ACETATE, AND 0.1 M CACODYLATE, CITRATE OR MES BUFFERED AT PH 5.5-6.0 |
